The Dounce Tissue Grinder is designed to retain a high percentage of cell nuclei and mitochondria in soft tissues or from cell cultures, and is very useful in enzyme studies. In operation, the pestle ball is encircled in liquid, which avoids heat buildup. The tube has a large reservoir and pouring lip and is supplied with a "loose" pestle and a "tight" pestle. The initial grinding is performed using the loose pestle. The grinding process is completed using the tight pestle. This grinder is used by moving the pestle up and down, and works best with cell suspensions or very soft tissue. Tight pestle specification: 0.001" to 0.003" (0.025 to 0.076mm). Loose pestle specification: 0.0035" to 0.0055" (0.089 to 0.14mm). The tissue grinders are made from Wheaton 33® low extractable borosilicate glass (meets ASTM Type I Class A and USP Type I standards). All sizes supplied two per case.