Automation Tips for Agilient Platforms
Experience the difference that Biotix proprietary technologies can bring to your high throughput screening assays. Biotix 384 automation tips features include: FlexFit, Delta Filter, X-Resin, Blade and Statix.
QUALITY TESTING
RNase/Dnase
Products are washed in distilled water and concentrated via centrifugation. Samples are added to previously established nucleic acid standards, incubated for 1 hour at 37 C, and tested on 2% gel using electrophoresis. Products must show no degradation of standards to pass.
Nucleic Acid
Products are washed in distilled water and concentrated via centrifugation. Then, samples are added to protocol specified PCR reactions and thermal cycled for 50 cycles. A 2% agarose gel electrophoresis is used to examine experimental samples, positive controls and negative controls. To pass, product samples must show no DNA amplification. Test sensitivity is 10 ng.
Endotoxin/Pyrogen
Products are tested for endotoxins by using the Limulus Amebocyte Lysate (LAL) gel assay according to FDA guidelines. Test sensitivity is 0.06 EU/ml.
Trace Metal
Products are washed in distilled water. The sample is then tested using reflectometry using a single strip test for the following metals: Ca, Cu, Fe, K, Mg, Mn and Ni. Standard solutions are used as positive controls. A reader is used to detect metals to a sensitivity of 500 mg/L.
PCR Inhibitor
Products are tested via PCR amplification and gel electrophoresis analysis. Samples must show normal amplification to be considered free of PCR inhibitors
Sterililzation
Products are sterilized using electron beam irradiation
CV Test
Each lot of Biotix product is CV tested and the resulting CV is then printed on the pack label. The volume tested is equal to the maximum volume per tip size
TIR - Total Indicated Runout
Biotix pipette tips are tested to ensure that they meet the SBS standards. Individual products specify their compatibility with 96, 384 or 1536-well plates
PROPRIETARY TECHNOLOGIES
- Statix - Static reduction and prevent technology (Patent Pending)
- X-Resin - Proprietary resin, virgin polypropylene
- Delta Filter - Color change filter (Patents Pending)
- Blade - Minimal distal end orifice (Patents Pending)
PIPETTOR COMPATIBILITY :
- Agilent Velocity 11 VPrep with 384 head
- Agilent Velocity 11Bravo with 384 head
