Accuris® PR2121-L-500 qMax Probe One-Step RT-qPCR Kit, Low Rox, 500 Reactions
Item #PR2121-L-500 Mfg #PR2121-L-500
- RNA to cDNA to qPCR in one tube
- High purity enzyme formulation for enhanced stability and performance
- Blue dye facilitates pipetting and visualization in plates
- Accuris Hot-Start Taq allows for preparation at room temperature
qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.
Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.
Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.
All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.